polyclonal goat anti mouse igg alexa fluor 488  (SouthernBiotech)

Journal: Science Advances

Article Title: E7 11-19 placement and orientation dictate CD8 + T cell response in structurally defined spherical nucleic acid vaccines

doi: 10.1126/sciadv.aec3876

Figure Lengend Snippet: ( A ) Schematics of three SNA designs presenting the HLA-A2–restricted HPV16 E7 11-19 peptide: E-SNA (peptide encapsulated in the liposomal core), N-HSNA (peptide displayed on the SNA surface via N-terminal DNA conjugation), and C-HSNA (peptide displayed via C-terminal conjugation). DNA-peptide conjugates were synthesized using a SPDP linker and a site-specific additional cysteine residue at either the N or C terminus of the peptide. ( B ) Zeta potential measurements comparing SNA formulations with liposomes. ( C ) Number mean size distribution of SNAs and liposomes measured by DLS. Data represent mean ± SD ( n = 3). ( D ) Cryo–electron microscopy (cryo-EM) images of E-SNA, N-HSNA, and C-HSNA. Particle size quantification was performed on at least 50 particles per group. Scale bars, 50 nm.

Article Snippet: The directly conjugated antibodies used for PDOTS staining were CD45-AlexaFluor-647 (BioLegend, HI30), EGFR-AbBy Fluor-594 (BioLegend, AY13), and HPV16 E7-AlexaFluor-488 (Bioss).

Techniques: Conjugation Assay, Synthesized, Residue, Zeta Potential Analyzer, Liposomes, Cryo-Electron Microscopy, Cryo-EM Sample Prep